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KMID : 0613820090190060809
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Journal of Life Science
2009 Volume.19 No. 6 p.809 ~ p.817
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Construction and Verification of Useful Vectors for Ectopic Expression and Suppression of Plant Genes
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Lee Young-Mi
Seok Hye-Yeon
Park Hee-Yeon
Park Ji-Im
Han Ji-Sung
Bang Tae-Sik
Moon Yong-Hwan
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Abstract
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The phenotypes associated with a gene function are often the best clue to its role in the plant. Transgenic plants ectopically expressing or suppressing a gene can provide useful information related to the gene function. In this study, we constructed three vectors - pFGL571, pFGL846 and pFGL847 - for the Agrobacterium-mediated ectopic expression of plant genes using pPZP211 and modified CaMV 35S, UBQ3 or UBQ10 promoters. The three vectors have several merits such as small size, high copy in bacteria, enough restriction enzyme sites in multi cloning sites and nucleotide sequence information. Analysis of transgenic plants containing GUS or sGFP reporter genes under the control of modified CaMV 35S, UBQ3 or UBQ10 promoter revealed that all of the three promoters showed high activities during most developmental stages after germination and in floral organs. Furthermore, we generated a RNAi module vector, pFGL727, to suppress plant gene expressions and confirmed that pFGL727 is useful for the suppression of a gene expression using rice transgenic plants. Taken together, our new vectors would be very useful for the ectopic expression or the suppression of plant genes.
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KEYWORD
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Plant, ectopic expression, suppression, promoters, RNAi
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